The most efficient, gentle method available for lysing bacterial E.coli Cell s
B-PER Bacterial Protein Extraction Reagent, first introduced in January 1998, provides the most efficient yet gentle method of extracting proteins, especially recombinant proteins, from bacteria (E. coli ). This extremely easy-to-use cell lysis solution utilizes a mild, nonionic detergent (proprietary) in 20 mM Tris•HCl (pH 7.5), eliminating the need for mechanical cell disruption. In fact, yields obtained with B-PER Reagent greatly exceed those obtained using standard sonication methods!
How does B-PER Bacterial Protein Extraction Reagent work?
Simply add B-PER Reagent to a bacterial pellet and shake for 10 minutes. The cell will be disrupted and the protein of interest gently, safely released. B-PER Reagent may be used for both soluble and insoluble protein extraction and inclusion body purification from total bacterial cell lysates. Soluble proteins can be recovered by pelleting cell debris; inclusion bodies can then be purified from the pellet using your optimized procedure. The novel composition of B-PER Reagent provides versatility for different applications and eliminates exogenous contamination of the recombinant protein by the lysis reagent. Depending on the particular application, additional components such as lysozymes, protease inhibitors, salts, reducing agents, chelating agents, etc. may be added to B-PER Reagent. If necessary, the nonionic detergent component can be easily removed by dialysis.
* GFP = Green Fluorescent Protein
E. Coli expressing GFP was extracted five times with B-PER Reagent or PBS/sonication. Each extraction was analyzed by SDS-PAGE
E. Coli expressing GFP was extracted five times with B-PER Reagent or PBS/sonication. Each extraction was analyzed by GFP activity assay.
Easy-to-use, single solution format
Rapid cell lysis of recombinant E.coli is complete in just 10 minutes
Achieves greater yields than possible with standard sonication methods
Flexible format that will work with any scale protein extraction
Designed for both soluble and insoluble protein extraction
Purifies inclusion bodies to near homogenous levels (does not solubilize inclusion bodies)