文中作者阐述了使用Wes nano-immunoassay method进行此项临床研究的原因：
1.The recent development of a method based on the combination of capillary nano-electrophoresis with immunoassay (CNIA), also known as Simple Western, requires only very small amounts of sample to be able to measure protein expression 3,7. This technical advance makes it possible to analyze the expression of 50-100 proteins in a single MM sample.
2.Here we present a method for the accurate and robust quantification of the expression of multiple proteins extracted from CD138-purified multiple myeloma samples frozen in RLT Plus buffer, which is commonly used for nucleic acid preservation and isolation
3.This makes the CNIA platform a fast, effective and accurate tool for exploring the impact of different proteins on MM survival and for investigating new protein biomarkers that could help predict the response to new drugs that directly target specific proteins.